Antinematodal activity of some Malaysian plant extracts against the pine wood nematode,Bursaphelenchus xylophilus

Abstract To investigate in vitro nematicidal activity against the pine wood nematode, Bursaphelenchus xylophilus, plant methanolic extracts were obtained from 26 medicinal plants and herbs in Vietnam. Of the plant extracts tested, a 10 mg ml−1 concentration of Cinnamomum cassia bark extract showed the highest level of nematicidal activity against juvenile and adult B. xylophilus after treatment for 7 h. The remainder of the plant extracts had little or no effect on juveniles or adults. Four solvents, n-hexane, chloroform, ethyl acetate and n-butanol, were used initially to partition the compounds obtained from C. cassia extracts. The compound (5 mg ml−1) in the n-hexane fraction showed the highest nematicidal activity at 60 min after treatment. Compounds III and IV were then separated from the n-hexane fraction using silica gel plates. These compounds showed very similar activity against the pine wood nematode. However, treatment with different concentrations of these compounds resulted in a significant difference in their effects on the mortality of the pine wood nematode, with greater concentrations leading to increased mortality after the same exposure time. Overall, treatment with 3 mg ml−1 of compounds III and IV resulted in greater than 95% mortality against juvenile and adult B. xylophilus at 50 min after treatment.

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Effects of inter‐specific crossbreeding between the invasive pine wood nematode, Bursaphelenchus xylophilus and native B . mucronatus on morphology and reproduction of the hybrid offspring

Forest Pathology ◽

10.1111/efp.12676 ◽

2021 ◽

Author(s):

Marek Tomalak ◽

Anna Filipiak

Keyword(s):

Bursaphelenchus Xylophilus ◽

Pine Wood Nematode ◽

Pine Wood ◽

Hybrid Offspring

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Influence of fungi on multiplication and distribution of the pine wood nematode, Bursaphelenchus xylophilus, in axenized Pinus thunbergii cuttings

Nematology ◽

10.1163/156854105776186343 ◽

2005 ◽

Vol 7 (6) ◽

pp. 809-817 ◽

Cited By ~ 3

Author(s):

Kazuo Suzuki ◽

Daisuke Sakaue ◽

Toshihiro Yamada ◽

Yu Wang

Keyword(s):

Population Size ◽

Early Stage ◽

Bursaphelenchus Xylophilus ◽

Pinus Thunbergii ◽

Disease Development ◽

Pine Wood Nematode ◽

Pine Wood ◽

Inoculation Site ◽

Fungal Hyphae ◽

Close Relationship

AbstractInfluence of fungi on multiplication and distribution of the pine wood nematode (PWN), Bursaphelenchus xylophilus, was investigated in Pinus thunbergii cuttings. Axenized nematodes and/or one of two fungi isolated from healthy and PWN-killed P. thunbergii were inoculated together into autoclaved cuttings. A close relationship between the existence and distribution of fungal hyphae, and the multiplication and distribution of PWN was observed. The PWN did not multiply when only axenized nematodes were inoculated in the absence of fungi. When fungi were present, PWN population size increased markedly. The number of nematodes was high at sites where fungal hyphae were distributed. It is suggested that the restriction of a large portion of the nematode population near the inoculation site during the early stage of disease development is closely related to restricted distribution of fungal hyphae.

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Larvicidal and Nematicidal Activities of 3-Acylbarbituric Acid Analogues against Asian Tiger Mosquito, Aedes albopictus, and Pine Wood Nematode, Bursaphelenchus xylophilus

Molecules ◽

10.3390/molecules22071196 ◽

2017 ◽

Vol 22 (7) ◽

pp. 1196 ◽

Cited By ~ 1

Author(s):

Seon-Mi Seo ◽

Hyo-Rim Lee ◽

Ji-Eun Lee ◽

Yong-Chul Jeong ◽

Hyung-Wook Kwon ◽

...

Keyword(s):

Aedes Albopictus ◽

Bursaphelenchus Xylophilus ◽

Pine Wood Nematode ◽

Asian Tiger Mosquito ◽

Pine Wood ◽

Asian Tiger ◽

Tiger Mosquito

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The effect of feeding behavior of Monochamus alternatus (Coleoptera: Cerambycidae) on the departure of pine wood nematode, Bursaphelenchus xylophilus (Nematoda: Aphelenchoididae)

10.1101/2019.12.13.875575 ◽

2019 ◽

Cited By ~ 1

Author(s):

Yang Wang ◽

Fengmao Chen ◽

Lichao Wang ◽

Lifeng Zhou ◽

Juan Song

Keyword(s):

Oxidative Phosphorylation ◽

Feeding Behavior ◽

Bursaphelenchus Xylophilus ◽

Pine Wood Nematode ◽

Monochamus Alternatus ◽

Pine Wood ◽

Start Date ◽

Significant Difference ◽

Departure Date ◽

Effect Of Feeding

AbstractIn order to study the causes of pine wood nematode (PWN) departure from Monochamus alternatus, the effects of the feeding behavior of M. alternatus on the start date of the departure of PWN were studied. The start date of the departure of PWN carried by the directly fed M. alternatus was 5—13 d after beetle emergence, mainly concentrated within 6—10 d, with a mean (±SD) of 8.02 ± 1.96 d. The start date of the departure of PWN carried by the M. alternatus fed after starvation was 5—14 d after beetle emergence, mainly concentrated within 6—9 d, with a mean of 7.76 ± 2.28 d. The results show that there was no significant difference in the start departure date of PWN between the two treatments. This shows that the feeding behavior of M. alternatus is not the trigger for PWN departure. At the same time, it was found that the motility of the PWN carried by M. alternatus at 8 d after emergence was significantly greater than that of the PWN carried by the newly emerged M. alternatus. And the PWN carried by M. alternatus at 8 d after emergence was extracted more easily than the PWN carried by newly emerged beetles. These results show that greater motility was associated with easier departure of PWN from M. alternatus. In addition, transcriptome sequencing found that the level of oxidative phosphorylation metabolism of PWN carried by beetles at 8 d after emergence was significantly higher than that in the PWN carried by newly emerged beetle. High oxidative phosphorylation was associated with increased energy production and motility by the PWN and were the internal cause of the start of nematode departure.

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Whole-organism Transcriptomic Insight Into the Effects of Cytco, A Novel Nematotoxic Protein on the Pine Wood Nematode Bursaphelenchus Xylophilus

10.21203/rs.3.rs-148830/v1 ◽

2021 ◽

Author(s):

Ye Chen ◽

Xiang Zhou ◽

Kai Guo ◽

Sha-Ni Chen ◽

Xiu Su

Keyword(s):

Enrichment Analysis ◽

Transcriptome Profiling ◽

Bursaphelenchus Xylophilus ◽

Control Measures ◽

Effective Control ◽

Large Set ◽

Pine Wood Nematode ◽

Pine Wood ◽

Protein Toxin ◽

Insight Into

Abstract Background: The pine wood nematode Bursaphelenchus xylophilus is a worldwide destructive pest on Pinus trees and lacks effective control measures. Screening nematotoxic protein toxins has been conducted to develop new strategies for nematode control. Results: The present study provided initial insights into the responses of B. xylophilus exposed to a nematocidal cytolytic-like protein (CytCo) based on the transcriptome profiling. A large set of differentially expressed genes (1266 DEGs) were found related to nematode development, reproduction, metabolism, motion, and immune system. In response to the toxic protein, B. xylophilus upregulated DEGs encoding cuticle collagens, transporters, and cytochrome P450. In addition, many DEGs related to cell death, lipid metabolism, major sperm proteins, proteinases/peptidases, phosphatases, kinases, virulence factors, and transthyretin-like proteins were downregulated. And Gene Ontology enrichment analysis showed that CytCo treatment significantly affecting DEGs functioning in muscle contraction, lipid localization, MAPK cascade. The pathway richness of Kyoto Encyclopedia of Genes and Genomes showed that the DEGs were concentrated in lysosome and fatty acid degradation. The weight co-expression network analysis indicated that the hub genes affected by CytCo were associated with the nematode cuticular collagen. Conclusions: These results showed that the CytCo protein toxin could interference gene expression to produce multiple nematotoxic effects, providing initial insight into its control potential of pine wood nematode.

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